Dot-blot
A Dot-Blot is a technique used to detect and identify targeted DNA on a membrane. In a dot-blot the target DNA to be detected is not separated by electrophoresis. Instead, a mixture containing the target DNA is applied directly on a membrane as a dot, and then is spotted through circular templates directly onto the membrane or paper substrate. T...
A Dot-Blot is a technique used to detect and identify targeted DNA on a membrane. In a dot-blot the target DNA to be detected is not separated by electrophoresis. Instead, a mixture containing the target DNA is applied directly on a membrane as a dot, and then is spotted through circular templates directly onto the membrane or paper substrate. This differs from a Southern blot because DNA samples are not separated electrophoretically. This is then followed by detection using nucleotide probes. The technique offers significant savings in time as compared to chromatography or gel electrophoresis, since the complex blotting procedures for the gel are not required. Dot blots can confirm the presence or absence of a biomolecule (target DNA) which can be detected by the DNA probe. Because a dot-blot does not require complicated instruments to operate, dot-blot assays are easy to use to detect the presence of foreign microorganisms in plant tissues.
Dot-blot There are 20 products.
Subcategories
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10-HYB artichoke CERT
Product components DOT BLOT DNA hybridization kit Non-radiactive probes 1 vial nylon membrane 5x10 cm, Spotted controls 1 negative 10 positive AILV-ARLV-AMCV-BYMV-CMV-PZSV-TICV-TMV-TSWV-TUMV 39 Tests code: H-MA-10
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10-HYB tomato CERT
Product components DOT BLOT DNA hybridization kit Non-radiactive probes 1 vial nylon membrane 5x10 cm, Spotted controls 1 negative 10 positive AMV-CMV-PepMV-PVX-PVY-PZSV-TIC-TMV-TSWV-TYLC 39 Tests code: H-MT-10
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4-HYB artichoke CERT
Product components Simultaneous detection of 4 artichoke viruses chosen by the customer Please select 4 from: AILV, AMCV, ARLV, BYMV, CMV, PZSV, TICV, TMV,TSWV, TUMV 45 Tests code: H-MA-4
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4-HYB tomato CERT
Product components Simultaneous detection of 4 tomato viruses chosen by the customer Please select 4 from: AMV, CMV, INSV, PepMV, PVX, PVY, PZSV, TICV, TMV,TSWV,TYLC 45 Tests code: H-MT-4
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AILV HYB (Artichoke Italian Latent Virus)
Product components DOT BLOT DNA hybridization kit Non-radiactive probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 negative 1 positive 48 Tests code: H-11
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AMCV HYB (Artichoke Mottled Crinkle Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 negative, 1 positive 48 Tests code: H-13
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AMV HYB (Alfalfa Mosaic Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-01
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ArLV HYB (Artichoke Latent Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-12
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BYMV HYB (Bean Yellow Mosaic Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-14
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CMV HYB (Cucumber Mosaic Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-02
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INSV HYB (Impatiens Necrotic Spot Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-03
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PepMV HYB (Pepino Mosaic Virus)
Product components Non-radioactive DNA probe 1 vial Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative 48 Tests code: H-10
