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Dot-blot

A Dot-Blot is a technique used to detect and identify targeted DNA on a membrane. In a dot-blot the target DNA to be detected is not separated by electrophoresis. Instead, a mixture containing the target DNA is applied directly on a membrane as a dot, and then is spotted through circular templates directly onto the membrane or paper substrate. T...

A Dot-Blot is a technique used to detect and identify targeted DNA on a membrane. In a dot-blot the target DNA to be detected is not separated by electrophoresis. Instead, a mixture containing the target DNA is applied directly on a membrane as a dot, and then is spotted through circular templates directly onto the membrane or paper substrate. This differs from a Southern blot because DNA samples are not separated electrophoretically. This is then followed by detection using nucleotide probes. The technique offers significant savings in time as compared to chromatography or gel electrophoresis, since the complex blotting procedures for the gel are not required. Dot blots can confirm the presence or absence of a biomolecule (target DNA) which can be detected by the DNA probe. Because a dot-blot does not require complicated instruments to operate, dot-blot assays are easy to use to detect the presence of foreign microorganisms in plant tissues.

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