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INSV HYB (Impatiens Necrotic Spot Virus)
New product
Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-03
More info
Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive labeled DNA probes patent n° 0001355079, University of Bari, Italy
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Impatiens Necrotic Spot virus (INSV) in tomato leaves
Specificity:
Sequence homology > 90% de Haan, P., de Avila, A.C., Kormelink, R., Westerbroek, A., Gielen, J.J., Peters, D. and Goldbach, R., 1992. The nucleotide sequence of the S RNA of Impatiens necrotic spot virus, a novel tospovirus. FEBS Lett. 306 (1), 27-32.
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of INSV PCR products; negative control of healthy tomato plant sap.
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.
