Dot blot hybridization by non-radioactive labeled DNA probe patent n° 0001355079 of 20.02.2009
Diagnostic signal:
Chemiluminescent by the action of alkaline phosphatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Artichoke Latent Virus (ArLV) in artichoke leaves
Specificity:
Rana, G.L., Russo, M., Gallitelli, D. and Martelli, G.P. (1982). Ann. appl. Biol. 101: 279. - Lumia V., Pasquini G., Barba M., 2003. Sensitive Detection of Artichoke Latent Virus in Globe Artichoke Field Samples by One-step RT-PCR or Tissue Imprint Hybridization. Journal of Phytopathology 151 (7-8), 477–479.
Supplied controls:
Spotted on the testing membrane 5x10 cm, positive control consists of ArLV PCR products, negative control of healthy artichoke plant sap
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months stored at 20°C
Sensitivity:
Approximately 20 μg of infected tissue
.