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TSWV HYB (Tomato Spotted Wilt Virus)
Nouveau produit
Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-07
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Data Sheet
Method of assay:
Dot blot hybridization non-radioactive labeled DNA Probe Patent n° 0001355079 of 20.02.2009, University of Bari, Italy
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Tomato Spotted Wilt Virus (TSWV) in tomato or artichoke leaves
Specificity:
Sequence homology > 90% Ohnishi,J., Kirita,M., Hosokawa,D. and Tsuda,S., 1997. Sequence analysis for M RNA segment of tomato spotted wilt tospovirus isolated in Japan. Ann. Phytopathol. Soc. Jpn. 63, 277.
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of TSWV PCR products; negative control of healthy artichoke tomato plant sap.
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.
