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PVX HYB (Potato Virus X)
Nouveau produit
Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-05
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Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive labeled DNA probe Project funded to Agritest by Apulia Region Government, PRAI Biotecnologie, 2004. Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus"
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Potato Virus X (PVX) in tomato leaves
Specificity:
Sequence homology > 90% Kraev,A.S., Morozov,S.Y., Lukasheva,L.I., Rozanov,M.N., Chernov,B.K., Simonova,M.L., Golova,Y.B., Belzhelarskaya,S.N., Pozmogova,G.E., Skryabin,K.S. and Atabekov,J.G., 1988. Primary structure and organization of the genome of potato virus X. Dokl. Akad. Nauk SSSR 300 (3), 711-716. Kavanagh,T., Goulden,M., Santa Cruz,S., Chapman,S., Barker,I. and Baulcombe,D., 1992. Molecular analysis of a resistance-breaking strain of potato virusX. Virology 189 (2), 609-617
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of PVX PCR products; negative control of healthy tomato plant sap.
Supplied reagents:
Non-radiactive DNA probe for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue
