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PZSV HYB (Pelargonium Zonate Spot Virus)
Nouveau produit
Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-06
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Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive labeled DNA probes Patent n° 0001355079, University of Bari, Italy
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Pelargonium Zonate Spot Virus (PZSV) in artichoke or tomato leaves
Specificity:
Sequence homology > 90% Finetti-Sialer, M. and Gallitelli, D., 2003. Complete nucleotide sequence of Pelargonium zonate spot virus and its relationship with the family Bromoviridae. J. Gen. Virol. 84 (Pt 11), 3143-3151.
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of PZSV PCR products; negative control of healthy artichoke or tomato plant sap.
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.
