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TMV HYB (Tobacco Mosaic Virus)
Nouveau produit
Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-09
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Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive labeled DNA Probes Project funded to Agritest by Apulia Region Government, PRAI Biotecnologie, 2004. Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus" "
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Tobacco Mosaic Virus (TMV) in tomato or artichoke leaves
Specificity:
Sequence homology > 90% Holt,C.A. and Beachy,R.N., 1991. In vivo complementation of infectious transcripts from mutant tobacco mosaic virus cDNAs in transgenic plants. Virology 181 (1), 109-117
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of TMV PCR products; negative control of healthy tomato plant sap
Supplied reagents:
Non-radioactive DNA probes for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.
