Method of assay:
Dot blot hybridization by non-radioactive labeled DNA Probes
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Cucumber Mosaic Virus (CMV) in artichoke or tomato leaves
Specificity:
Sequence homology > 90% Rizzo,T.M. and Palukaitis,P., 1988. Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA. J. Gen. Virol. 69 (Pt 8), 1777-1787.
Supplied controls:
Spotted on the testing membrane positive control consists of CMV PCR products; negative one of healthy tomato or artichoke plant sap.
Supplied reagents:
Non-radioactive DNA probes for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.