ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies or hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme ...
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies or hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate, to produce a measureable product. The most crucial element of the detection strategy is a highly-specific antibody-antigen interaction. ELISAs are typically performed in 96-well (or 384-well) polystyrene plates, which will passively bind antibodies and proteins. It is this binding and immobilization of reagents that makes ELISAs so easy to design and perform. Having the reactants of the ELISA immobilized to the microplate surface makes it easy to separate bound from non-bound material during the assay. This ability to wash away nonspecifically bound materials makes the ELISA a powerful tool for measuring specific analytes within a crude preparation.
Product components DAS ELISA KIT Plum Pox Virus Capture polyclonal – conjugate polyclonal - positive and negative controls 500 tests code: K-21B 1000 tests code: K-21A
Product components DASI ELISA KIT Plum Pox Virus Cherry Capture polyclonal - detection monoclonal line AC - conjugate antimouse - positive and negative controls 500 tests code: K-14B 1000 tests code: K-14A
Product components DASI ELISA KIT Plum Pox Virus Dideron Capture polyclonal - detection monoclonal line 4DG5 - conjugate antimouse - positive and negative controls 500 tests code: K-12B 1000 tests code: K-12A
Product components DASI ELISA KIT Plum Pox Virus El Amar Capture polyclonal - detection monoclonal line EA24 - conjugate antimouse - positive and negative controls 500 tests code: K-13B 1000 tests code: K-13A
Product components DASI ELISA Plum Pox Virus Marcus Capture polyclonal - detection monoclonal line AL - conjugate antimouse - positive and negative controls 500x5 tests code: K-11B 1000x5 tests code: K-11A
Product components DASI ELISA KIT Plum Pox Virus Capture polyclonal - detection monoclonal line 5B - conjugate antimouse - positive and negative controls 500x5 tests code: K-10B 1000x5 tests code: K-10A
Standard formulation for ELISA according to Clark and Adams (1977). No preservative added.
Assay type: DAS ELISA using polyclonal antiserum from rabbit.
Assay type: DAS ELISA using polyclonal antiserum from rabbit.
Potato Black Ringspot Nepovirus. PBRSV (Andean Potato Calico Strain of Tobacco Ringspot Virus, Potato Calico Strain of Tobacco Ringspot Virus) Assay type: DAS ELISA using polyclonal antiserum from rabbit.