GCMV Elisa (Grapevine Crown Mosaic Virus)

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Plum Pox Virus-Cherry (PPV Cherry)

Condition: New product

Product components

DASI ELISA KIT Plum Pox Virus Cherry

Capture polyclonal - detection monoclonal line AC - conjugate antimouse - positive and negative controls

500 tests code: K-14B

1000 tests code: K-14A

More details

More info

Data Sheet

Method of assay:

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AC produced by DIBCA, University of Bari, Italy

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Plum Pox Virus type Cherry (PPV C) in leaf of stone fruit tree and Nicotiana

Specificity:

Myrta A., Potere O., Crescenzi A., Nuzzaci M. and Boscia D.. 2000. Properties of two monoclonal antibodies specific to the cherry strain of plum pox virus. Journal of Plant Pathology 82 (2), 95-101

Supplied controls:

1 Positive: freeze dried Nicotiana benthamiana leaf tissue tested for PPV C - 1 Negative: freeze-dried peach leaf The presence (positive control) and the absence (negative control) of the virus was ascertained by indexing, ELISA and PCR

Supplied reagents:

Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C.