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Plum Pox Virus-Cherry (PPV Cherry)

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Product components
DASI ELISA KIT Plum Pox Virus Cherry
Capture polyclonal - detection monoclonal line AC - conjugate antimouse - positive and negative controls
500 tests code: K-14B
1000 tests code: K-14A

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Data Sheet
Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AC produced by DIBCA, University of Bari, Italy
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Plum Pox Virus type Cherry (PPV C) in leaf of stone fruit tree and Nicotiana
Specificity:
Myrta A., Potere O., Crescenzi A., Nuzzaci M. and Boscia D.. 2000. Properties of two monoclonal antibodies specific to the cherry strain of plum pox virus. Journal of Plant Pathology 82 (2), 95-101
Supplied controls:
1 Positive: freeze dried Nicotiana benthamiana leaf tissue tested for PPV C - 1 Negative: freeze-dried peach leaf The presence (positive control) and the absence (negative control) of the virus was ascertained by indexing, ELISA and PCR
Supplied reagents:
Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C.

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