GCMV Elisa (Grapevine Crown Mosaic Virus)

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Plum Pox Virus-Marcus (PPV Marcus)

Condition: New product

Product components

DASI ELISA Plum Pox Virus Marcus

Capture polyclonal - detection monoclonal line AL - conjugate antimouse - positive and negative controls

500x5 tests code: K-11B

1000x5 tests code: K-11A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AL produced by DIBCA, University of Bari, Italy

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Plum Pox Virus type Marcus (PPV M) in leaf of stone fruit tree

Specificity:

Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M.T., Myrta A., Di Terlizzi B. and Savino V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.European Journal of Plant Pathology 103, 447-480

Supplied controls:

1 Positive - 1 Negative Freeze-dried peach leaf. The presence and the absence of PPV–M were ascertained by indexing on peach indicator, ELISA and PCR

Supplied reagents:

Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C.