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Plum Pox Virus-Marcus (PPV Marcus)

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Product components
DASI ELISA Plum Pox Virus Marcus
Capture polyclonal - detection monoclonal line AL - conjugate antimouse - positive and negative controls
500x5 tests code: K-11B
1000x5 tests code: K-11A

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Data Sheet
Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AL produced by DIBCA, University of Bari, Italy
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Plum Pox Virus type Marcus (PPV M) in leaf of stone fruit tree
Specificity:
Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M.T., Myrta A., Di Terlizzi B. and Savino V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.European Journal of Plant Pathology 103, 447-480
Supplied controls:
1 Positive - 1 Negative Freeze-dried peach leaf. The presence and the absence of PPV–M were ascertained by indexing on peach indicator, ELISA and PCR
Supplied reagents:
Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C.

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