GCMV Elisa (Grapevine Crown Mosaic Virus) View larger

PepMV HYB (Pepino Mosaic Virus)

New product

Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-10

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CAD $226.00

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Data Sheet
Method of assay:
Dot blot hybridization with non-radioactive labeled DNA Probes Project funded to Agritets by Apulia Region Government, PRAI Biotecnologie, 2004. "Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus"
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Pepino Mosaic Virus (PepMV) in tomato leaves
Specificity:
Sequence homology > 90% Aguilar,J.M., Hernandez-Gallardo,M.D., Cenis,J.L., Lacasa,A. and Aranda,M.A, 2002. Complete sequence of the Pepino mosaic virus RNA genome. Arch. Virol. 147 (10), 2009-2015
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of PepMV PCR products. Negative control consists of healthy tomato plant sap.
Supplied reagents:
Non-radioactive DNA probes for 50 reactions
Shelf life:
12 months at –20 °C.
Sensitivity:
Approximately 20 ug of infected tissue

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