Method of assay:
Dot blot hybridization with non-radioactive labeled DNA Probes Project funded to Agritets by Apulia Region Government, PRAI Biotecnologie, 2004. "Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus"
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
Molecular detection of Pepino Mosaic Virus (PepMV) in tomato leaves
Sequence homology > 90% Aguilar,J.M., Hernandez-Gallardo,M.D., Cenis,J.L., Lacasa,A. and Aranda,M.A, 2002. Complete sequence of the Pepino mosaic virus RNA genome. Arch. Virol. 147 (10), 2009-2015
Spotted on the testing membrane 5 x 10 cm. Positive control consists of PepMV PCR products. Negative control consists of healthy tomato plant sap.
Non-radioactive DNA probes for 50 reactions
12 months at –20 °C.
Approximately 20 ug of infected tissue