Method of assay:
Dot blot hybridization by non-radioactive labeled DNA probe patent n° 0001355079 of 20.02.2009, University of Bari, Italy
Chemiluminescent by the action of alkaline phosphatase on the CSPD or CDP star substrate
Number of tests:
Molecular detection of Artichoke Mottled Crinkle Virus (AMCV) in artichoke leaves
Grieco, F. and Gallitelli, D. (1990). Nucl. Acids Res. 18: 1300.
Spotted on the testing membrane 5x10 cm positive control consists of AMCV PCR products negative control consists of healthy artichoke plant sap
Non-radioactive DNA probe for 50 reactions
12 months stored at -20°C
Approximately 20 μg of infected tissue.