Method of assay:
Dot blot hybridization with non radioactive labeled DNA probes
Chemiluminescent, by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
Simultaneous molecular detection of n°4 viruses in artichoke leaves
artichoke phytosanitary certification
Sequence homology > 90% See references for each of the chosen virus
1 membrane 5 x 10 cm, spotted with positive and negative controls. 4 positive controls consist of PCR products per each virus; 1 negative control of health artichoke plant sap.
DNA probes liquid
12 months when stored at –20 °C.
Approximately 20 μg of infected tissue