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TYLCV HYB (Tomato Yellow Leaf Curl Virus)
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Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-08
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Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive labeled DNA Probe Project funded to Agritest by Apulia Region Government, PRAI Biotecnologie, 2004. Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus"
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Tomato Yellow Leaf Curl Viruse (TYLCV) in tomato leaves
Specificity:
Sequence homology > 90% Accotto,G., Navas-Castillo, J., Noris, E., Moriones, E., and Louro, D., 2000. Typing of Tomato yellow leaf curl viruses in Europe. European Journal of Plant Pathology 106: 179-186.
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consists of TYLCV PCR products; negative control of healthy tomato plant sap.
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months –20 °C
Sensitivity:
Approximately 20 μg of infected tissue.
