Method of assay:
Dot blot hybridization with non radioactive labeled DNA probes
Diagnostic signal:
Chemiluminescent, by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
45
Intended Use:
Simultaneous molecular detection of 4 viruses in tomato leaves
Tomato phytosanitary certification
Specificity:
Sequence homology > 90% See references for each of the chosen virus
Supplied controls:
1 membrane 5 x 10 cm, spotted with positive and negative controls. 4 positive controls consist of PCR products per each virus; 1 negative control of health tomato plant sap.
Supplied reagents:
DNA probes liquid
Shelf life:
12 months when stored at –20 °C.
Sensitivity:
Approximately 20 μg of infected tissue.