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AMV HYB (Alfalfa Mosaic Virus)
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Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-01
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Data Sheet
Method of assay:
Dot blot hybridization by non-radioactive DNA probe patent n° 0001355079 of 20.02.2009, University of Bari, Italy
Diagnostic signal:
Chemiluminescent by the action of alkaline phosphatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Alfalfa Mosaic Virus (AMV) in artichoke leaves
Specificity:
Sequence homology > 90% Barker,R.F., Jarvis,N.P., Thompson,D.V., Loesch-Fries,L.S. and Hall,T.C., 1983. Complete nucleotide sequence of alfalfa mosaic virus RNA3. Nucleic Acids Res. 11 (9), 2881-2891.
Supplied controls:
Spotted on the testing membrane 5x10 cm positive control consists of AMV PCR products, negative of healthy artichoke plant sap
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months stored at -20°C
Sensitivity:
Approximately 20 μg of infected tissue.
