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PVY HYB (Potato Virus Y)
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Product components
Non-radioactive DNA probe 1 vial
Nylon membrane 5x10 cm, spotted controls, 1 positive, 1 negative
48 Tests code: H-04
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Data Sheet
Method of assay:
Dot blot hybridization with non-radioactive labeled DNA probes Project funded to Agritest by Apulia Region Government, PRAI Biotecnologie, 2004. Sviluppo della diagnostica molecolare vegetale per la realizzazione di kit diagnostici per i fitovirus"
Diagnostic signal:
Chemiluminescent by the action of alkaline phoshatase on the CSPD or CDP star substrate
Number of tests:
48
Intended Use:
Molecular detection of Potato Virus Y (PVY) in tomato leaves
Specificity:
Sequence homology > 90% Dhar, A.K., Singh, R.P. and Boucher, A., 1994. Molecular cloning and sequencing of the capsid and the nuclear inclusion protein genes of a North American PVY N isolate. Can. J. Microbiol. 40 (9), 798-804.
Supplied controls:
Spotted on the testing membrane 5 x 10 cm. Positive control consist of PVY PCR products; negative control of healthy tomato plant sap.
Supplied reagents:
Non-radioactive DNA probe for 50 reactions
Shelf life:
12 months at –20 °C
Sensitivity:
Approximately 20 μg of infected tissue
