GCMV Elisa (Grapevine Crown Mosaic Virus)

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Grapevine LeafRoll associated Virus 2 (GLRaV-2)

Condition: New product

Product components

DAS ELISA KIT Grapevine Leafroll associated Virus 2

Capture polyclonal – conjugate monoclonal - positive and controls

500 tests code: K-04B

1000 tests code: K-04A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich (DAS) by monoclonal antibody line R19, produced by DIBCA, University of Bari, Italy

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Grapevine Leafroll associated Virus 2 (GLRaV -2) in phloem, mature leaf and petiols. Not for sample of leaf from American Vitis and their hybrids

Specificity:

Zhou Z., Abou-Ghanem N, Boscia D., Potere O., Goszczynski D.E. and Castellano M.A., 2000. Monoclonal antibodies for detection and characterization of grapevine leaf-roll associated virus 2. Abstracts 13th Meeting of ICVG, Adelaide 2000 Boscia D., Greif C., Gugerli P., Martelli G.P., Walter B., and Gonsalves D., 1995. Nomenclature of grapevine leaf-roll associated putative closterovirus. Vitis 34:171-175 Abou Ghanem-Sabanadzovic N, Sabanadzovic S., Castellano M. A., Boscia D. and Martelli G.P., 2000. Properties of a new isolate of grapevine leafroll-associated virus 2. Vitis 39: 119-121

Supplied controls:

Positive 2 wells/plate Negative 2 wells/plate - Freeze-dried grapevine phloem. The presence and the absence of GLRaV-2 were ascertained by indexing on Vitis indicators, by ELISA and PCR

Supplied reagents:

Capture 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C