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Grapevine LeafRoll associated Virus 2 (GLRaV-2)

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Product components
DAS ELISA KIT Grapevine Leafroll associated Virus 2
Capture polyclonal – conjugate monoclonal - positive and controls
500 tests code: K-04B
1000 tests code: K-04A

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Data Sheet
Method of assay:
ELISA Double Antibody Sandwich (DAS) by monoclonal antibody line R19, produced by DIBCA, University of Bari, Italy
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Grapevine Leafroll associated Virus 2 (GLRaV -2) in phloem, mature leaf and petiols. Not for sample of leaf from American Vitis and their hybrids
Specificity:
Zhou Z., Abou-Ghanem N, Boscia D., Potere O., Goszczynski D.E. and Castellano M.A., 2000. Monoclonal antibodies for detection and characterization of grapevine leaf-roll associated virus 2. Abstracts 13th Meeting of ICVG, Adelaide 2000 Boscia D., Greif C., Gugerli P., Martelli G.P., Walter B., and Gonsalves D., 1995. Nomenclature of grapevine leaf-roll associated putative closterovirus. Vitis 34:171-175 Abou Ghanem-Sabanadzovic N, Sabanadzovic S., Castellano M. A., Boscia D. and Martelli G.P., 2000. Properties of a new isolate of grapevine leafroll-associated virus 2. Vitis 39: 119-121
Supplied controls:
Positive 2 wells/plate Negative 2 wells/plate - Freeze-dried grapevine phloem. The presence and the absence of GLRaV-2 were ascertained by indexing on Vitis indicators, by ELISA and PCR
Supplied reagents:
Capture 200 µl/well - conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C

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