GCMV Elisa (Grapevine Crown Mosaic Virus)

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Grapevine LeafRoll associated Virus 1 (GLRaV-1)

Condition: New product

Product components

DAS ELISA KIT Grapevine Leafroll associated Virus 1

Capture polyclonal – conjugate polyclonal - positive and negative controls

500 tests code: K-03B

1000 tests code: K-03A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Grapevine Leafroll associated Virus 1 (GLRaV-1) in phloem, mature leaf and petiols. Not for sample of leaf from American Vitis and their hybrids

Specificity:

Boscia D., Greif C., Gugerli P., Martelli G.P., Walter B., and Gonsalves D., 1995. Nomenclature of grapevine leaf-roll associated putative closterovirus. Vitis 34:171-175 Gugerli,P. 1991. Grapevine closterovirus, p.40-51. In I.C. Rumbos, R. Bovey, D. Gonsalves, W.B. Hewitt, and G.P. Martelli (ed.), Proceedings of the 10th Meeting of the International Council for the Study of Viruses and Virus Diseases of the Grapevine (ICVG). Plant Protection Institute, P.O. Box 303, 38001 Volos, Greece

Supplied controls:

Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GLRaV-1 were ascertained by indexing on Vitis indicators, by ELISA and PCR

Supplied reagents:

Capture 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C