Method of assay:
ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Grapevine Leafroll associated Virus 1 (GLRaV-1) in phloem, mature leaf and petiols. Not for sample of leaf from American Vitis and their hybrids
Specificity:
Boscia D., Greif C., Gugerli P., Martelli G.P., Walter B., and Gonsalves D., 1995. Nomenclature of grapevine leaf-roll associated putative closterovirus. Vitis 34:171-175 Gugerli,P. 1991. Grapevine closterovirus, p.40-51. In I.C. Rumbos, R. Bovey, D. Gonsalves, W.B. Hewitt, and G.P. Martelli (ed.), Proceedings of the 10th Meeting of the International Council for the Study of Viruses and Virus Diseases of the Grapevine (ICVG). Plant Protection Institute, P.O. Box 303, 38001 Volos, Greece
Supplied controls:
Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GLRaV-1 were ascertained by indexing on Vitis indicators, by ELISA and PCR
Supplied reagents:
Capture 200 µl/well - conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C