Storage buffer |
50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA,
1mM DTT, 0.5mM PMSF , 50% glycerol.
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TnATaq DNA polymerase
New product
TnATaq DNA polymerase is a thermostable enzyme isolated from E. coli which encodes Taq DNA polymerase gene. This enzyme contains 5’-3’ poly merase and 5’-3’ exonuclease activity.
More info
Cat No |
Pack size |
Conc. |
| TAMB01Z-500 | 500U | 5U/uL |
| TAMB01Z-1000 | 1000U | 5U/uL |
| TAMB01Z-2500 | 2500U | 5U/uL |
PCR cycles program |
| Step | Temperature | Time | Cycle |
| Initial denaturation | 94-95ºC | 1-3 mins | 1 |
| Denaturation | 94-95ºC | 10-60sec | 25-35 |
| Annealing | 50-68ºC | 10-30sec | |
| Extension | 72ºC | 1min/1kb | |
| Final extension | 72ºC | 1-10 mins | 1 |
IMPORTANT: Annealing temperature should be 2-6ºC lower than the primer melting temperature.
10X reaction buffer |
Containing 15mM MgCl2
Unit description |
One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl2, 200uM each of dATP, dCTP, dGTP, dTTP, 10 mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 μl.
Storage buffer |
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at -20ºC, 100 mM KCl, 0.1 mM EDTA.
Source |
E coli clone
Quality control |
The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. SDS/PAGE – 95 kD band. Activity and stability tested via thermo-cycling. The error rate per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 10-4. Estimated half life at 95ºC is 0.5 hours.
Application |
- Amplification of DNA fragments up to 5 kb
- Labelling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
- Cycle sequencing
- Generation of PCR product for TA cloning