Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by polyclonal antibodies
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Prunus Necrotic Ringspot Virus (PNRsV) in leaf of pome or stone fruit tree
Specificity:
Cochran,L.C. and Hutchins,L.M. (1941). Phytopathology 31B 860
Supplied controls:
1 Positive and 1 Negative Freeze-dried peach leaf. The presence and the absence of PNRsV were ascertained by indexing of peach indicators and ELISA
Supplied reagents:
Capture 200 µl/well - detection 200 µl/well - conjugate protein A 200 µl/well
Shelf life:
9-12 months when stored at 4°C.