GCMV Elisa (Grapevine Crown Mosaic Virus)

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Plum Pox Virus-Universal (PPV Universal)

Condition: New product

Product components

DASI ELISA KIT Plum Pox Virus

Capture polyclonal - detection monoclonal line 5B - conjugate antimouse - positive and negative controls

500x5 tests code: K-10B

1000x5 tests code: K-10A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 5B supplied by Instituto Valenciano de Investigaciones Agrarias (IVIA), Spagna

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-10000

Intended Use:

In vitro detection of Plum Pox Virus (PPV) in leaf of stone fruit tree

Specificity:

Cambra M., Asensio M., Gorris M.T., Perez E., Camarasa E., Garcia J.A.,Moya J.J., Lopez-Abella D., Vela C., and Sanz A.,1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bulletin 24, 569-577

PPV cherry, Dideron, El Amar, Marcus, Recombinant, Winona (internal data 2011)

Supplied controls:

1 Positive- 1 Negative Freeze-dried peach leaf. The presence and the absence of PPV were ascertained by indexing on peach indicator, ELISA and PCR

Supplied reagents:

Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C.