GCMV Elisa (Grapevine Crown Mosaic Virus)

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Plum Pox Virus-Dideron (PPV Dideron)

Condición: Nuevo producto

Product components

DASI ELISA KIT Plum Pox Virus Dideron

Capture polyclonal - detection monoclonal line 4DG5 - conjugate antimouse - positive and negative controls

500 tests code: K-12B

1000 tests code: K-12A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 4DG5 supplied by Instituto Valenciano de Investigaciones Agrarias (IVIA), Spagna

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Plum Pox Virus type Dideron (PPV D) in leaf of stone fruit tree

Specificity:

Cambra M., Asensio M., Gorris M.T., Perez E., Camarasa E., Garcia J.A., Moya J.J., Lopez-Abella D., Vela C., and Sanz A., 1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bulletin 24, 569-577

Supplied controls:

1 Positive - 1 Negative Freeze-dried peach leaf. The presence and the absence of PPV –D were ascertained by indexing on peach indicator, ELISA and PCR

Supplied reagents:

Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life:

9-12 months when stored at 4°C.