Method of assay:
ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of in young leaf of Plum Bark Necrosis Stem Pitting-associated Virus (PBNSPaV) in stone fruit tree
Specificity:
Boscia D., O. Potere, M.A. Castellano and V. Savino, 2006. A new antiserum for the detection of plum necrosis stem pitting –associated virus by mean of ELISA.
Supplied controls:
1 Positive - 1 Negative Freeze-dried peach leaf tissue. The absence and the presence of PBNSPaV were ascertained by indexing on peach indicators, PCR and ELISA.
Supplied reagents:
Capture 200 µl/well - Conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C.