GCMV Elisa (Grapevine Crown Mosaic Virus)

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Grapevine Virus A (GVA)

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Product components

DAS ELISA KIT Grapevine Virus A

Protein A - capture polyclonal – conjugate monoclonal line F5 - positive and negative controls

1000 tests code: K-07B

2000 tests code: K-07A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich (DAS) by monoclonal antibody line F5 produced by DIBCA, University of Bari, Italy

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Grapevine Virus A (GVA) in grapevine phloem

Specificity:

Boscia D., E. Aslouj, V. Elicio, V. Savino, M.A. Castellano and G.P. Martelli, 1992. Production,characterization and use of monoclonal antibodies to grapevine virus A. Archives of Virology 127, 184-194

Supplied controls:

Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GVA were ascertained by indexing on Vitis indicators, by ELISA and PCR

Supplied reagents:

Pre-coating Proteina A 100 µl/well - capture 100 µl/well - conjugate 100 µ/well

Shelf life:

9-12 months since the packaging date when stored at 4°C

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