Xylella fastidiosa

Method of assay

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

Diagnostic signal

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 or 1000

Intended Use   

in vitro detction of Xylella fastidiosa (Wells and Raju) in olive tree leaves

Agritest test performances available in Appendix 1 of the OEPP/EPPO PM7/24 (2) Xylella fastidiosa

Specificity          

X. fastidiosa subsp. fastidiosa, multiplex, pauca

Polyclonal antibodies raised against subsp. pauca strain CoDiRO

Giampetruzzi, A., Chiumenti, M., Saponari, M., Donvito, G., Italiano, A., Loconsole, G., Boscia, D., Cariddi, C., Martelli, G. P. and Saldarelli, P. 2015. Draft genome sequence of Xylella fastidiosa CoDiRO strain. Genome Announc 3(1):e01538-14. doi: 10.1128/genomeA.01538-14. http://genomea.asm.org/content/3/1/e01538-14.full.pdf+html

No ELISA cross reaction found against the plant pathogens:

Agrobacerium tumefaciens biovars 1, 2, A.vitis; Brenneria populi, B. quercina, B. rubrifaciens; Burkholderia andropogonis; Clavibcter michiganensis subsp. michiganensis; Erwinia amylovora, Pantoea agglomerans; Pantoea stewartii subsp stewartii; Pseudomonas amygdali, P. marginalis pv marginalis, P. savastanoi pv savastanoi , P. s. pv syringe, P. s. pv garcae; Ralstonia solanacearum; Xanthomonas arboricola pv celebensis, X. a. pv corylina, X. a. pv juglandis, X. a. pv pruni; Xanthomonas campestris pv citri, X. c. pv populi, X. c. pv vesicatoria, X. c. pv viticola, X. hortorum pv pelrgonii  Data produced by CREA-PAV, Rome, Italy.

Supplied controls

1 positive freeze-dried inactivated bacteria extract - 1 negative freeze-dried healthy olive leaf extract

Supplied reagents

Capture antibodies 200 ul/well - conjugate antibodies 200 ul/well

Shelf life

9-12 months when stored at 4°C

Sensitivity

10^5-10^4 cfu/ml PBS