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Plum Pox Virus-El Amar (PPV El Amar)

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Product components
DASI ELISA KIT Plum Pox Virus El Amar
Capture polyclonal - detection monoclonal line EA24 - conjugate antimouse - positive and negative controls
500 tests code: K-13B
1000 tests code: K-13A

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Data Sheet
Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line EA24 produced by DIBCA, University of Bari, Italy
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Plum Pox Virus type El Amar (PPV EA) in leaf of stone fruit tree
Specificity:
Myrta A., Potere O., Boscia D., Candresse T., Cambra M., Savino V. 1998. Production of a monoclonal antibody specific to the El Amar strain of plum pox virus. Acta Virologica 42, 248-250
Supplied controls:
1 Positive - 1 Negative Freeze-dried peach leaf. The presence and the absence of PPV –EA were ascertained by indexing on peach indicator, ELISA and PCR
Supplied reagents:
Capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well
Shelf life:
9-12 months when stored at 4°C

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