Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line GBG11B (MAB 2) produced by DIBCA, University of Bari, Italy. Antigen direct binding
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Grapevine Virus B (GVB) in grapevine phloem
Specificity:
Bonavia M., Digiaro M., Boscia D., Boari A., Bottalico G., Savino V., and Martelli G.P., 1996. Studies on “corky rugose wood” of grapevine and on diagnosis of grapevine virus B. Vitis 35, 53-58
Supplied controls:
Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GVB were ascertained by indexing on Vitis indicators, by ELISA and PCR
Supplied reagents:
Detection 200 µ/well - conjugate 200 µ/well
Shelf life:
9-12 months when stored at 4°C