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Grapevine Fleck Virus (GFkV)

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Product components
DASI ELISA KIT Grapevine Fleck Virus
Capture polyclonal- detection monoclonal line 117 – conjugate anti mouse - positive and negative controls
500 tests code: K-09B
1000 tests code: K-09A

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Data Sheet
Method of assay:
ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 117, produced by DPPMA, University of Bari, Italy
Diagnostic signal:
Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Number of tests:
500-1000
Intended Use:
In vitro detection of Grapevine Fleck Virus (GFkV) in grapevine phloem and young leaf
Specificity:
Boscia D., V. Elicio, V. Savino and G.P. Martelli, 1994. Production of monoclonal antibodies to grapevine fleck virus. Plant Pathology 44, 160-163
Supplied controls:
Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GFkV were ascertained by indexing on Vitis indicators, by ELISA and PCR
Supplied reagents:
Coating Pab 200 µl/well Revealing Mab 200 µ/well Conjugate Anti mouse – AP 200 µl/well
Shelf life:
9-12 months when stored at 4°C

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