GCMV Elisa (Grapevine Crown Mosaic Virus)

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Grapevine Fleck Virus (GFkV)

Condition: New product

Product components

DASI ELISA KIT Grapevine Fleck Virus

Capture polyclonal- detection monoclonal line 117 – conjugate anti mouse - positive and negative controls

500 tests code: K-09B

1000 tests code: K-09A

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Data Sheet

Method of assay:

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 117, produced by DPPMA, University of Bari, Italy

Diagnostic signal:

Colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Number of tests:

500-1000

Intended Use:

In vitro detection of Grapevine Fleck Virus (GFkV) in grapevine phloem and young leaf

Specificity:

Boscia D., V. Elicio, V. Savino and G.P. Martelli, 1994. Production of monoclonal antibodies to grapevine fleck virus. Plant Pathology 44, 160-163

Supplied controls:

Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GFkV were ascertained by indexing on Vitis indicators, by ELISA and PCR

Supplied reagents:

Coating Pab 200 µl/well Revealing Mab 200 µ/well Conjugate Anti mouse – AP 200 µl/well

Shelf life:

9-12 months when stored at 4°C

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